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比较了在OP50-NGM、JM109-NGM、OP50-LB、JM109-LB四种不同培养基上秀丽隐杆线虫的生长效果;测定了在三种温度和含不同甘油浓度冻存液中的虫体保存效果。结果显示,秀丽隐杆线虫在OP50-NGM培养基中生长良好,在JM109-LB培养基中生长较差。在-80℃最佳温度条件下,秀丽隐杆线虫在含50mL/L甘油的LB培养基中的存活率达78%;在含50mL/L甘油的PBS中的存活率达52%;而在含100mL/L甘油的水溶液中的存活率仅为8%。用液氮保存,成虫和幼虫均可存活;而在-80℃和-20℃条件下,仅有幼虫存活。结果表明,秀丽隐杆线虫在OP50-NGM培养基中培养发育生长较好;在-80℃条件下,含50mL/L甘油的LB培养基适于保存秀丽隐杆线虫。
Abstract：Growth effect of Caenorhabditis elegans on four different medium including OP50-NGM,JM109-NGM,OP50-LB and JM109-LB were evaluated and compared with each other.The preserving effects of C.elegans in three preserving solutions,with different proportional glycerol were evaluated under the condition of liquid nitrogen-80℃ and-20℃,respectively.Results showed that C.elegans grew better on the NGM medium covered with E.coli OP50,than other three mediums.For preserving solution,C.elegans had a survival rate of 78% in LB medium containing 50mL/L glycerol at-80℃ and it had a survival rate of 52% in PBS at the same condition as LB medium,while in water with 100mL/L glycerol,the survival rate was only 8%.Both adults and larvae can survive in liquid nitrogen,but only the larvae can survive at-80℃and-20℃.In conclusion,C.elegans grew well on NGM medium covered with E.coli OP50,and the optimum preserving condition was LB medium with 50mL/L glycerol at-80℃.
[1]赵晴,蒋湉湉.秀丽隐杆线虫研究综述[J].安徽农业科学,2010,38(19):10092-10095.ZHAO Qing,JIANG Tian-tian.Reviewing of Caenorhabditiselegans[J].Journal of Anhui A gricultural Sciences,2010,38(19):10092-10095.(in Chinese)
[2]沈静德.秀丽隐杆线虫是研究发育生物学的好材料[J].生物学通报,1991(9):18,20.SHEN Jing-de.Caenorhabditis elegans,a good material ofstudying developmentalbiology[J].Bulletin of Biology,1991(9):18,20.(in Chinese)
[3]刘恩岐,仓林让.模型动物——秀丽隐杆线虫研究进展[J].动物科学与动物医学,2003,20(10):110-112.LIU En-qi,CANG Lin-rang.Animal mode-lreviewing Cae-norhabditis elegans[J].Animal Science and Animal Medicine,2003,20(10):110-112.(in Chinese)
[4]KENYON C.The nematode Caenorhabditis elegans[J].Sci-ence,1988,240(4858):1448-1452.
[5]WILLIAMS P L,DUSENBERY D B.Using thenematodeCae-norhabditi elegans to predict mammalian acute lethality tome-tallic salts[J].Toxicol Ind Health,1988,4(4):469-478.
[6]KENNEY S J,ANDERSON G L,WILLIAMS P L,et al.Per-sistence of Escherichia coli O157:H7,Salmonella newport,and Salmonella poona in the gut of a free-living nematode,Caenorhabditis elegans,and transmission to progeny and unin-fected nematodes[J].Int J Food Microbiol,2005,101(2):227-236.
[7]HASHMI S,HASHMI G,GAUGLER R.Genetic transforma-tion of an entomopathogenic nematode by microinjection[J].JInvertebr Pathol,1995,66(3):293-296.
[8]BRENNER S.The genetics of Caenorhabditis elegans[J].Ge-netics,1974,77(1):71-94.
[9]WARD S,BURKE D J,SULSTON J E,et al.Genomic organ-ization of major sperm protein genes and pseudogenes in thenematode Caenorhabditis elegans[J].J Mol Biol,1988,199(1):1-13.
[10]SULSTON J,HODGKIN J.Methods in the Nematode Cae-norhabditi Elegans[M].New York:Cold Spring Harbor La-boratory Press,1988:587-606.
[11]庞林海,杜爱芳,李孝军,等.秀丽隐杆线虫培养特性与保存方法研究[J].浙江农业学报,2007,19(1):34-36.PANG Lin-hai,DU A-ifang,LI Xiao-jun,et al.Study on cul-ture and preservation of Caenorhabditis elegans[J].Acta Ag-riculturae Zhejiangensis,2007,19(1):34-36.(in Chinese)
[12]杜爱芳,庞林海,李孝军,等.秀丽隐杆线虫(Caenorhabditiselegans)培养特性研究(初报)[C]//中国畜牧兽医学会家畜寄生虫学分会第五次代表大会暨第八次学术研讨会论文集.桂林,2004:345-347.DU A-ifang,PANG Lin-hai,LI Xiao-jun,et al.Study on char-acteristics of cultivation of Caenorhabditis elegans(First Re-port)[C]//The Proceedings of the 5th Congress and 8th Con-ference of the Branch of Livestock Parasitology of ChineseAssociation of Animal and Veterinary Sciences.Guilin,2004:345-347.(in Chinese)
基本信息:
DOI：10.16656/j.issn.1673-4696.2011.10.002
中图分类号:Q95-33
引用信息:
[1]李有全,关贵全,彭欲率,等.秀丽隐杆线虫的培养与保存研究[J].中国兽医科学,2011,41(10):1001-1004.DOI:10.16656/j.issn.1673-4696.2011.10.002.
基金信息:
国家肉牛产业技术体系项目(CARS-38);; 国家自然科学基金项目(30800820);; 欧盟项目EPIZONE(FOOD-CT-2006-016236)